ROLE OF EMERICELLA NIDULANS AND EPICOCCUM NIGRUM IN ‎CONTROLLING SUGER BEET LEAF SPOT DISEASE ‎

Document Type : Original articles

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Sugar Crops Research Institute, Agricultural Research Centre, Giza, Egypt ‎

Abstract

SYMMARY
 Two fungal isolates from commercial sugar beet phyllospher in Kafr-Elshaikh governorate, Egypt were tested for its antagonistic activity against Cercospora beticola the causal pathogen of sugar beet cercosporia leaf spot (CLS) disease in vitro.
Three methods were used to evaluate its antagonistic activity (Conventionalstreak, culture filtrates and volatile gases). The ability of the tested isolates to produce PR-proteins and growth-promoter indole acetic acid were studied. The tested isolates Emericella nidulans (Aspergillus nidulans) and Epicoccum nigrum found to have the potential to produce chitinase and β-1, 3 glucanase while Emericella   nidulansthe only found to have the potential to produce indole acetic acid (IAA). The tested fungal isolates showed a remarkable antagonistic activity in both green-house and open field against C. beticola compared with the healthy control and the infected control.
Also it was found that the control efficiency of the two isolates was almost equivalent to the tested fungicide. The present data show that phyllospheric fungi can play as a good candidate to be used as a bio-control agent against sugar beet leaf spot disease.

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